Enzyme standard
double wavelength detection is relative to the enzyme standard instrument for
single wavelength detection, reduce the determination of interference and
circuit interference, so the test results more accurate.
Microplate reader in a single wavelength determination spectrophotometry, except when determination by endogenous interference (including noise and drift, voltage, etc) outside factors, the liquid surface tension has a very big impact. In the testing process, because the surface tension of liquid surface effect, the surface of the liquid is not a plane, but to form a concave, seemingly concave lens from the side, this will inevitably affect the normal through the light path. Because concave influence of the liquid, the light in through the liquid surface, in addition to the normal is part of liquid absorption outside, still have part is reflected and reflection (such as light through the concave lens that way), affect absorbency detection. And the standard of instrument uses the enzyme is through the optical fiber transmission of point light source, if every time can at the same position detection, absorbency repetitive will get a guarantee, but due to mechanical and other sports errors can't be sure every holes are in the same part of the testing, so caused the hole and there is a certain difference between hole.
Microplate reader double wavelength detection, reduce the determination of interference and circuit interference, so the determination results improved obviously. Because of the different liquid surface tension, leading to a single wavelength much error determination. And with different detergent can affect the last to join the substrate and terminated after fluid of liquid surface, the collection and collation of the surfactant with join detergent and cleaning of the formation of the liquid surface more concave, single wavelength detection of more influence, and the concentration of the surfactant is priced. And with neutral distilled water after washing, single and double wavelength detection technology results are basically the same.
In the enzyme league immune method for the determination of antigens, antibodies, commonly used marker enzyme horseradish peroxidase by use of the substrate is generally adjacent benzene 2 amine or four methyl , show color after termination, both in 630 and 655 nm in nm spectrophotometry value only in absorption peaks place (492 nm / 450 nm) absorbency value below 1%, therefore, the use of dual wavelength detection, will not affect the detection sensitivity. Were suggested in enzyme league immune test, enzyme standard than color should first instrument double wave. It increases the critical analysis of the specimen in accuracy, reduce the error.
For microplate reader double wavelength detection domestic manufacturers--perlong with advanced technology of medical pace, after continuous technology research and development, also produced to the wavelength detection of enzyme standard instrument. Including medical equipment is very popular in the customer high praise in the industry of the enzyme standard instrument 9602, enzyme standard instrument 9602 A, enzyme standard instrument 9602 G, etc.
Perlong medical treatment will be in "loving the life, health care" business philosophy and the tenet, and improve the technical level and the function of equipment, innovate, to benefit people. If all of the dual wavelength detection perlong medical enzyme standard instrument price interested, can call toll-free number 400-6656888 or click on page on the right side of the "customer service online" ask questions!
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