Biochemistry analyzer method
types commonly used methods end point method, the continuous monitoring
method, turbidity than method, according to the detection principle for
material choice one analysis method.
First method is called again, the equilibrium method, is based on the reaction to balance the reaction products absorption spectral characteristics and the size of the intensity of light absorption of material of a class of quantitative analysis method, a little end point method and the two end two kinds of method. A method is to use the characteristics of one or two kind of reagent, when for the content and reagents reaction to the end, determination of mixed solution spectrophotometry to calculate the concentration of the articles to be tested, the method of commonly used a total protein double shrink urea method, albumin bromine cresol green method, the glucose oxidase method, manual operation most of the methods are a little end point method. The law also says two fixed time method, if is single reagents analysis when the wavelength interfering substances with determination the absorption spectra of the overlap, through choosing two end point method can eliminate the disturbance caused blank samples, the analysis process is in the samples and reagents mixed after A DuanYan demurrage incurred after reading A point A1, A2 after A certain amount of time to read, and then compare standards and determination of the Δ A (Δ A = A2-A1) value, get to the concentration of the object under test. Creatinine GuWeiSuan method is a typical example of single reagents two points. If is double reagent analysis, choose two points in addition to eliminate the analysis method of the interference caused by blank samples, still can eliminate endogenous interfering substances interference, the analysis process was to join the reagent after 1 read A1, A2 after 2 to join reagent read, read blank sample equivalent to A1, A2 is actual color rendering reaction, then compare standards and determination of the Δ A (Δ A = A2-A1) value, get to the concentration of the object under test. In order to improve the accuracy of the test method, choose the method should be set when the end point of zero readings, sample analysis parameters such as blank two, the former is in the reaction before the start reading, can be deducted before the reaction mixture of reagents and sample blank readings; The latter is the sample and blank reagent have been spectrophotometry, reaction required a cup than color.
Second, the continuous monitoring method and dynamic analysis, says rate method and so on, is the basic principle in the enzymatic reaction of the optimum condition, with physics, chemistry or enzymatic reaction analysis method, the response rate constant regular (zero level reaction period) inside continuous observation and records must be reaction time substrates and product the quantity change, with the unit the enzyme reaction time velocity of the size of the enzyme calculated and the concentration of the metabolites. Specific methods have two rate method and more rate method: two rate method is through the observation in zero level two time points during the period of reaction of the absorbency, with two points of difference spectrophotometry (Δ A) divided by time (points), calculated the absorbency changes every minute value; More rate method is zero order reaction in every time period (2-30 s) on a monitor, and continuous monitoring many times, seek the unit time speed of response, this kind of method and can be divided into the least square method, more δ method, regression method, take rate time method, etc. The method has obvious advantages of analysis is greatly improve the speed and accuracy, mainly is suitable for the enzyme activity and its metabolites is determined. In the continuous monitoring process, even if not add samples, reagent the substrates would also automatically degradation get a result, so should be set reagent blank rate, different batches of reagent reagent blank rate, its value is measured in water sample and project results to determine the results shall be deducted reagent blank rate of values.
Three, than turbidity method to be automatic biochemistry analyzer general can only do transmission immune than turbidity analysis, when light through a certain volume contains immune complex solution, as the solution of existing antigen-antibody complex particle of light reflection and absorption, cause the transmission light decreases, determination of the flux and antigen antibody is inversely proportional to the amount of the complex. It is often used in end-point technique, mainly used in serum protein of special testing, such as apolipoprotein, trace protein, acute opposite should protein, immunoglobulin and some drug monitoring, etc. But if the sample for the high concentration of antigen, antigen and antibody formation of immune complex molecular will become smaller, and vulnerable to disintegrate, make the turbidity it down, so the immune than turbidity analysis process must be set in the area before the check, comparative analysis in the process after the difference of two readings point, if a little a bit after than before spectrophotometry is low, it means the antigen has excess, should be diluted the sample weight.
First method is called again, the equilibrium method, is based on the reaction to balance the reaction products absorption spectral characteristics and the size of the intensity of light absorption of material of a class of quantitative analysis method, a little end point method and the two end two kinds of method. A method is to use the characteristics of one or two kind of reagent, when for the content and reagents reaction to the end, determination of mixed solution spectrophotometry to calculate the concentration of the articles to be tested, the method of commonly used a total protein double shrink urea method, albumin bromine cresol green method, the glucose oxidase method, manual operation most of the methods are a little end point method. The law also says two fixed time method, if is single reagents analysis when the wavelength interfering substances with determination the absorption spectra of the overlap, through choosing two end point method can eliminate the disturbance caused blank samples, the analysis process is in the samples and reagents mixed after A DuanYan demurrage incurred after reading A point A1, A2 after A certain amount of time to read, and then compare standards and determination of the Δ A (Δ A = A2-A1) value, get to the concentration of the object under test. Creatinine GuWeiSuan method is a typical example of single reagents two points. If is double reagent analysis, choose two points in addition to eliminate the analysis method of the interference caused by blank samples, still can eliminate endogenous interfering substances interference, the analysis process was to join the reagent after 1 read A1, A2 after 2 to join reagent read, read blank sample equivalent to A1, A2 is actual color rendering reaction, then compare standards and determination of the Δ A (Δ A = A2-A1) value, get to the concentration of the object under test. In order to improve the accuracy of the test method, choose the method should be set when the end point of zero readings, sample analysis parameters such as blank two, the former is in the reaction before the start reading, can be deducted before the reaction mixture of reagents and sample blank readings; The latter is the sample and blank reagent have been spectrophotometry, reaction required a cup than color.
Second, the continuous monitoring method and dynamic analysis, says rate method and so on, is the basic principle in the enzymatic reaction of the optimum condition, with physics, chemistry or enzymatic reaction analysis method, the response rate constant regular (zero level reaction period) inside continuous observation and records must be reaction time substrates and product the quantity change, with the unit the enzyme reaction time velocity of the size of the enzyme calculated and the concentration of the metabolites. Specific methods have two rate method and more rate method: two rate method is through the observation in zero level two time points during the period of reaction of the absorbency, with two points of difference spectrophotometry (Δ A) divided by time (points), calculated the absorbency changes every minute value; More rate method is zero order reaction in every time period (2-30 s) on a monitor, and continuous monitoring many times, seek the unit time speed of response, this kind of method and can be divided into the least square method, more δ method, regression method, take rate time method, etc. The method has obvious advantages of analysis is greatly improve the speed and accuracy, mainly is suitable for the enzyme activity and its metabolites is determined. In the continuous monitoring process, even if not add samples, reagent the substrates would also automatically degradation get a result, so should be set reagent blank rate, different batches of reagent reagent blank rate, its value is measured in water sample and project results to determine the results shall be deducted reagent blank rate of values.
Three, than turbidity method to be automatic biochemistry analyzer general can only do transmission immune than turbidity analysis, when light through a certain volume contains immune complex solution, as the solution of existing antigen-antibody complex particle of light reflection and absorption, cause the transmission light decreases, determination of the flux and antigen antibody is inversely proportional to the amount of the complex. It is often used in end-point technique, mainly used in serum protein of special testing, such as apolipoprotein, trace protein, acute opposite should protein, immunoglobulin and some drug monitoring, etc. But if the sample for the high concentration of antigen, antigen and antibody formation of immune complex molecular will become smaller, and vulnerable to disintegrate, make the turbidity it down, so the immune than turbidity analysis process must be set in the area before the check, comparative analysis in the process after the difference of two readings point, if a little a bit after than before spectrophotometry is low, it means the antigen has excess, should be diluted the sample weight.
Article : From Perlong medical (http://www.pl999.net/)
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