Perlong medical equipment sales calcium is popular science indispensable human body of importance chemical element, the researchers through hematology analyzer after blood calcium is divided into research will YouLiGai and combined with calcium.
The calcium content and plasma protein binding albumin concentration related, such as the plasma albumin declined obviously, and the result is also reduce calcium, so that the serum total calcium content decreased, but for YouLiGai don't reduce, so the clinical symptoms appear not lack calcium.
(1) determination of calcium ions: ion calcium can use calcium ion selective electrode method.
(2) the total calcium determination: total blood calcium determination method mainly has atomic absorption spectrophotometry, dye combination and titration method, etc.
Among them is a more common application of complexometric titration, its advantage is easy operation, do not need special equipment, YongXieLiang less, accuracy, comply with the requirements. Indicator of commonly used a yellow green grain and calcium calcium red.
Atomic absorption spectrophotometry use air a acetylene flame, calcium absorption characteristics is the light of the flame 422.7 nm flame photometric method is high sensitivity, but not suitable for routine inspection. Ion selective electrode determination of calcium ion has set up a file in the clinical application.
Colorimetric method have methyl musk blue grass phenol method and adjacent armour phenolphthalein collaterals and legal.
Methyl musk blue grass phenol colorimetric principle:
In the serum calcium ion in alkaline solution and musk blue grass phenol (MTB) union, to generate a blue complex. Join the appropriate 8 a hydroxyl KuiLin, can eliminate magnesium ions on the determination of interference, and also deal with calcium compared the standard fluid, can seek the serum total calcium.
Article : From perlong medical (http://www.pl999.net)
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